Best way to represent an ELISA result is to report exact concentration as calculated against some standard by using OD values. That will represent the true difference. Sometimes, OD values can be confusing.
How do you analyze ELISA?
- Use a 4-parameter algorithm to generate the standard curve.
- Subtract background absorbance from all data points.
- Take into account dilution factors.
- Calculate average, standard deviation, and CV when running replicates.
How do you analyze qualitative ELISA data?
https://www.youtube.com/watch?v=QrnRkyUAA3g
How is quantitative ELISA interpreted?
Quantitative and Semiquantitative ELISA Test Results Quantifying the signal in each sample is performed by comparing the value to the standard curve to determine the concentration. It is also possible to determine relative quantification by comparing samples to each other or to a reference sample.