- Step 1: Connect the light microscope to a power source.
- Step 2: Turn the revolving nosepiece so the lowest objective lens is in position.
- Step 3: Mount your specimen onto the stage.
- Step 4: Use the metal clips to keep your slide in place.
How do you take a picture with a microscope?
- Using the microscope, examine the specimen by eye and select the area of interest and magnification required.
- Increase the light source to maximum intensity.
- Hold the camera lens against the microscope eyepiece.
- Use the camera's zoom function to increase the size of the circle as required.
How do you prepare and observe amicroscope slide?
https://www.youtube.com/watch?v=YU0DYrU_0FM
How do you prepare amicroscope slide for bacteria?
https://www.youtube.com/watch?v=RY5ulw1TIQo
How do you prepare a bacterial sample for staining?
In order to heat fix a bacterial smear, it is necessary to first let the bacterial sample air dry. Then either place the slide in the slide holder of a microincinerator, or pass the dried slide through the flame of a Bunsen burner 3 or 4 times, smear side facing up. Once the slide is heat fixed, it can then be stained.
How do you prepare amicroscope slide GCSE?
- Peel a thin, transparent layer of epidermal cells from the inside of an onion.
- Place cells on amicroscope slide.
- Add a drop of water or iodine (a chemical stain).
- Lower a coverslip onto the onion cells using forceps or a mounted needle. This needs to be done gently to prevent trapping air bubbles.
What are the five steps to using a microscope?
- Step 1: Moving Your Microscope. Carry the microscope with two hands.
- Step 2: Microscope Lens Care. Never touch any lens with your fingers.
- Step 3: Microscope Parts.
- Step 4: Prepare a Slide.
- Step 5: Insert the Slide.
- Step 6: Set Up for Viewing.
- Step 7: Light Control.
- Step 8: Focus the Microscope.
What are the steps for preparing bacterial cells for staining?
- Heat-fix the bacteria.
- Air-dry the bacteria.
- Create a bacterial smear.
What are the steps needed to prepare a bacterial smear and complete the Gram stain process?
- Apply a smear of bacteria on to a slide.
- Add about 5 drops of Hucker's Crystal Violet to the culture.
- Add about 5 drops of iodine solution to the culture.
- Tilt slide and decolorize with solvent (acetone-alcohol solution) until purple color stops running.
- Add about 5 drops of Safranine O.
What are the steps in preparing a wet mount slide?
- Collect a thin slice of your sample and place it on a clean, dry slide.
- Place one drop of water over your sample.
- Place the coverslip at a 45-degree angle with one edge touching the water and let go.
- Your slide is ready to be viewed.
How should prepared slides be handled?
To keep your prepared microscope slides in good condition, always store them in a container made for the purpose and away from heat and bright light. The ideal storage area isa cool, dark location, such as a closed cabinet in a temperature-controlled room. Stained slides naturally fade over time.
What are the 5 parts of a microscope?
- Eyepiece or Ocular Lens. Eyepiece lens magnifies the image of the specimen.
- Eyepiece Tube or Body Tube. The tube hold the eyepiece.
- Nosepiece.
- Objective Lenses.
- Arm.
- Stage.
- Stage Clips.
- Diaphragm (sometimes called the Iris)
What are the steps in using amicroscope to focus specimen?
To focus a microscope, rotate to the lowest-power objective, and place your sample under the stage clips. Play with the magnification using the coarse adjustment knob and move your slide around until it is centered.
What are the steps of a simple stain?
https://www.youtube.com/watch?v=8ODeT9DLHKI
How do you prepare amicroscope slide for a plant cell?