Thermo Scientific™ SPECTRONIC™ 200 is the standard for rugged, simple-to-use visible spectrophotometers for teaching applications.
What does a spectrophotometer measure?
Spectrophotometry is a standard and inexpensive technique to measure light absorption or the amount of chemicals in a solution. It uses a light beam which passes through the sample, and each compound in the solution absorbs or transmits light over a certain wavelength.
How do you use spectronic 20D+?
https://www.youtube.com/watch?v=20TpmeaTJho
What is quantitative spectrophotometry?
Spectrophotometry is the quantitative measurement of the interaction of ultraviolet (UV), visible, and infrared (IR) radiation with a material and has an impact on a wide field of science and technology.
What is a spec 20 used for?
The Spectronic 20, often called the Spec 20, is a spectrophotometer. A spectrophotometer measures the intensity of a light beam before and after it passes through a sample and compares these two intensities. The Spec 20 reports two types of measurements: percent transmittance and absorbance.
What is transmittance in spectrophotometer?
Transmittance is simply the percentage of light impinging on a solution that passes through the solution and emerges to be detected by the instrument.
What does a monochromator do in a spectrophotometer?
A monochromator produces a beam of light with an extremely narrow bandwidth, or light of a single color. It is used in optical measuring instruments where tunable monochromatic light is sought. A monochromator produces a beam of light with an extremely narrow bandwidth, or light of a single color.
How do you turn on a spectronic 200?
Plug the power line mains cord, provided with the instrument, into a properly earthed / grounded receptacle. Be sure to confirm that the power switch is turned OFF before plugging the power cable of the instrument into the receptacle. Switch ON the power to the spectrophotometer.
How do you use spectronic 200?
https://www.youtube.com/watch?v=1dAolBz7wfM
Why do we use the spectrum scan mode in the spec 200?
For the spectra of photosynthetic pigments, start by setting the Spec 200 to scan mode. That is the same mode described above, to find the absorption peak for riboflavin, so many of the directions here are identical to the directions given there.Sep 12, 2019
What does spectronic 200 measure?
Delivering quick, convenient measurements in the hands of novice users, the SPECTRONIC 200 spectrophotometer is the perfect fit for the teaching lab. The CCD detector measures each portion of the spectrum with the ideal integration time to account for lamp intensity and detector sensitivity.