PE-Cyanine7 is a tandem fluorophore that can be excited by a broad range of lasers including 488 nm, 532 nm, and 561 nm. Dye degradation is common if exposed to photobleaching, fixation, and higher temperatures. This is a bright dye often used in flow cytometry for low-abundance antigens.
What is a fluorochrome and how is it used?
Fluorescent dyes (or fluorochromes) are commonly used as detection reagents in various applications such as cellular imaging and flow cytometry. Fluorochromes absorb light energy of a specific wavelength and re-emit it at a longer wavelength. This fluorescence is the read-out signal provided by the instrument.
What is Cy5 used for?
Cy5 dye is a traditional far-red–fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications.
Can I use PE and PE Cy7 together?
In my experience, it is not advisable to use APC/Cy7 and PE/Cy7 together due to heavy cross-beam contamination as their emission max are exactly same. However, they can be used together if you are using machines like the Cytek Aurora.
What is cy7?
Cy®7 is a near-infrared (NIR) fluorophore commonly used in applications such as immunolabeling, fluorescence microscopy, and flow cytometry. Cy®7 is useful for in vivo imaging when tissues are observed in the near-infrared region. Cy® cyanine dyes are registered trademarks of GE Healthcare.
What is cyanine used for?
Cyanine dyes are used to label proteins, antibodies, peptides, nucleic acid probes, and any kind of other biomolecules to be used in a variety of fluorescence detection techniques: Flow cytometry, Microscopy (mainly Visible range, but also UV, IR), Microplate assays, Microarrays, as well as "light-up Probes," and in
What does Cy3 stand for?
Each sample is labeled with a different fluorescent dye (e.g., patient DNA with cyanine 3 (Cy3) and control DNA with cyanine 5 (Cy5)).
How do cyanine dyes work?
1 INTRODUCTION. Cyanine dyes form a J-aggregate showing an intense narrow absorption band shifted to longer wavelength relative to the monomer band and an almost Stokes-shift free fluorescence band. The J-aggregate can be regarded as two-dimensional system with a brickstone arrangement of the chromophores.
What is excited emission?
When a compound absorbs light (and hence energy), electrons are raised from the ground state to an excited state. Electrons return to the ground state by a variety of transitions which may involve the emission of a quantum of light (radiative transition).26 Feb 2020
How does excitation and emission work?
The emission maximum is chosen and only emission light at that wavelength is allowed to pass to the detector. Excitation is induced (usually by means of a monochromator) at various excitation wavelengths and the intensity of the emitted fluorescence is measured as a function of wavelength.
What is the difference between fluorescence and emission?
In fluorescence, the emission is basically immediate and therefore generally only visible, if the light source is continuously on (such as UV lights); while phosphorescent material can store the absorbed light energy for some time and release light later, resulting in an afterglow that persists after the light has been
What is excitation in spectroscopy?
The excitation spectrum is a range of light wavelengths that add energy to a fluorochrome, causing it to emit wavelengths of light, the emission spectrum2. Within a flow cytometer, the appropriate ranges of excitation and emission wavelengths of light are selected by bandpass filters.26 Feb 2020
What wavelength is PE?
PE (Ex-Max 496 nm/Em-Max 578 nm): R-phycoerythrin - is a pigment naturally found in red algae where it transfers light energy to chlorophyll during photosynthesis. PE has one of the largest absorption co-efficiencies making it one of the brightest fluorochromes available to date.