Promoterless vector for measuring the activity of promoter and enhancer sequences with a luciferase assay.
What is a luciferase assay?
A luciferase assay is used to determine if a protein can activate or repress the expression of a target gene. ... Luciferase is an enzyme used for bioluminescence by various organisms in nature, most famously the firefly.
Are luciferase Reporter vector?
The pGL3 Luciferase Reporter Vectors provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These may be cis- or trans-acting factors. ... The assay of this genetic reporter is rapid, sensitive and quantitative.
Is luciferase a plasmid?
Luciferase-containing plasmids are commonly used to investigate the effect of regulatory elements, such as promoters, enhancers and untranslated regions, or the effect of mutations of these regulatory elements on gene expression.
How do you perform luciferase assay?
Luciferase activity is measured using a Luminometer equipped with an automated dual injection system. A volume of 20 μl of cell lysate is transferred to a microcentrifuge tube and placed in the injection chamber.
How is luciferase detected?
In the luciferase reaction, light is emitted when luciferase acts on the appropriate luciferin substrate. Photon emission can be detected by light sensitive apparatus such as a luminometer or modified optical microscopes.
What is luciferase assay system?
The Luciferase Assay System is an extremely sensitive and rapid reagent for quantitation of firefly luciferase. Linear results are seen over at least eight orders of magnitude of enzyme concentration, and less than 10–20 moles of luciferase can be measured under optimal conditions.
Where do you find luciferase?
insect fireflies
What is luciferase reporter vector?
Panomics' Luciferase Reporter Vectors are designed to monitor transcription factor binding activity in vivo through the use of the standard luciferase assay. Each Luciferase Reporter Vector contains multiple repeats of a specific transcription factor binding element.
Is luciferase a vector?
The pGL3 Luciferase Reporter Vectors provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These may be cis- or trans-acting factors. ... The specific transcriptional characteristics of the pGL3 Vectors will vary for different cell types.
Is luciferase a reporter gene?
A commonly used reporter gene is the luciferase gene from the firefly Photinus pyralis. This gene encodes a 61-kDa enzyme that oxidizes D-luciferin in the presence of ATP, oxygen, and Mg(++), yielding a fluorescent product that can be quantified by measuring the released light.
Is a reporter a vector?
Reporter vectors contain a defining reporter gene, which can be used to detect the successful transformation of a gene of interest into a cell, as well the protein expression of that gene. ... Common reporters include fluorescence proteins (like GFP and RFP) or enzymes such as beta-galactosidasebeta-galactosidaseβ-galactosidase, also called lactase, beta-gal or β-gal, is a family of glycoside hydrolase enzymes that catalyzes the hydrolysis of β-galactosides into monosaccharides through the breaking of a glycosidic bond.https://en.wikipedia.org › wiki › Beta-galactosidaseBeta-galactosidase - Wikipedia and luciferase.
What type of protein is luciferase?
Fluorescent Proteins 101: Luciferase. Luciferases are a class of enzymes capable of catalyzing chemical reactions in living organisms resulting in the emission of photons.Jun 24, 2014
How do you test for luciferase activity?
To measure luciferase activity of the cell lysates, you will need a multiwell plate or a tube containing cell lysates and a luminometer. This apparatus detects the photon emission produced from the luciferase reaction and the unit of measurement comes out as relative light units (RLU).