Invitrogen RNAlater Stabilization Solution is an aqueous, nontoxic tissue RNA stabilization and storage reagent that rapidly permeates tissues to stabilize and protect cellular RNA. RNAlater solution minimizes the need to immediately process tissue samples or to freeze samples in liquid nitrogen for later processing.
Is RNA later toxic?
According to the manufacturer the lack of RNAlater toxicity makes the product user-friendly for sample transportation, but biological safety may still be a concern with some RNAlater-treated specimens. Picornavirus, rhabdovirus, and HIV in cell culture supernatants retain infectivity after RNAlater stabilization(5).Jul 1, 2007
How do you prepare RNA later?
To prepare samples for storage at –20°C, first incubate the samples in RNAlater Solution overnight at 4°C to allow thor- ough penetration of the tissue, then transfer to –20°C. Samples can subsequently be thawed at room temperature and refrozen without affecting the amount or the integrity of the recoverable RNA.
How do you dispose of RNA later?
Store RNAlater Solution at room temperature. If any precipitation of RNAlater Solution is seen, heat it to 37°C and agitate to redissolve it. RNAlater Solution can be safely discarded down the sink and flushed with water. RNAlater Solution is known to react with hypochlorite solu- tions, such as common bleach.
Does RNA later need to be refrigerated?
Before immersion in RNAlater® Solution, cut large tissue samples to ≤ 0.5 cm in any single dimension. Place the fresh tissue in 5–10 volumes of RNAlater® Solution. Most samples in RNAlater® Solution can be stored at room temperature for 1 week without compromising RNA quality, or at –20°C or –80°C indefinitely.
Does RNA later lyse cells?
Bacteria RNAlater is bacteriostatic; although bacteria do not grow in RNAlater, the cells remain intact. E. coli cells stored in RNAlater for 1 month at 2-8°C are intact and yield undegraded RNA. The cell types that have been evaluated do not lyse when frozen at –80 °C in RNAlater.
How does RNA later work?
RNAlater-ICE transitions tissue from a frozen to a non-frozen state. The frozen tissue is simply placed in RNAlater-ICE and left at -20°C overnight. Treated tissues can then be used directly in standard homogenization and isolation protocols and processed like fresh tissue.
Is RNA later a fixative?
Because RNAlater is readily used for field sample transportation, laboratory assistants could falsely interpret RNAlater as a disinfectant fixative. Therefore, RNAlater-treated specimens should be marked as potentially infective materials.Jul 1, 2007
How is RNA removed?
RNA can be separated from other cellular components by adding chloroform and centrifuging the solution. This separates the solution into two phases: organic and aqueous phases. The aqueous phase contains RNA.