Freeze cells in tubes from 1 to 5 mL using the Thermo Scientific™ Mr. Frosty™ Freezing Container. The simple-to-use system is designed to achieve a rate of cooling very close to -1°C/minute, the optimal rate for cell preservation.
How long can cells stay in Mr Frosty?
How long the mammalian cells can be stored at -80 ᵒC (in Mr Frosty) before transferring the cells in liquid nitrogen? Within a week, however even after months it can be done.
Why is isopropanol used in Mr Frosty?
The Mr. Frosty is filled with 200 ml of isopropanol at room temperature and the freezing vials containing the cells are placed in the container and the container is placed in the -80oC freezer. The effect of the isopropanol is to allow the tubes to come to the temperature of the freezer slowly, at about 1oC per minute.