Spectrophotometry is an experimental technique that is used to measure the concentration of solutes in a specific solution by calculating the amount of light absorbed by those solutes. [1] This technique is powerful because certain compounds will absorb different wavelengths of light at different intensities.
What is an example of spectrophotometry?
An example of an experiment in which spectrophotometry is used is the determination of the equilibrium constant of a solution. The amount of light that passes through the solution is indicative of the concentration of certain chemicals that do not allow light to pass through.
What are the two types of spectrophotometry?
Among the different types of spectrophotometry, there are two primary methods employed; absorption spectrophotometry, which is concerned with the absorption of radiation and specific spectra of light, and Ultraviolet-Visible Range spectrophotometry, which is concerned with the reflectance of specific spectra of a given Among the different types of spectrophotometry, there are two primary methods employed; absorption spectrophotometryabsorption spectrophotometryAbsorption spectroscopy refers to spectroscopic techniques that measure the absorption of radiation, as a function of frequency or wavelength, due to its interaction with a sample. The sample absorbs energy, i.e., photons, from the radiating field. The transmitted energy can be used to calculate the absorption.https://en.wikipedia.org › wiki › Absorption_spectroscopyAbsorption spectroscopy - Wikipedia, which is concerned with the absorption of radiation and specific spectra of light, and Ultraviolet-Visible Range spectrophotometry, which is concerned with the reflectance of specific spectra of a given
What is the function of a spectrophotometer?
An absorption spectrophotometer is a device used to measure absorbed light intensity as a function of wavelength. In UV–visible spectrophotometers, a beam of light from a suitable UV and/or visible light source is passed through a prism or diffraction grating monochromator.
What are the uses of a spectrophotometer?
A spectrophotometer is an analytical instrument used to quantitatively measure the transmission or reflection of visible light, UV light or infrared light. Spectrophotometers measure intensity as a function of light source wavelength.22 Dec 2021
What are the uses of spectrophotometer in laboratory?
Spectrophotometers measure light intensity as a function of wavelength and are commonly used to measure the concentration of a compound in an aqueous solution. Depending on the type of spectrophotometer, different wavelengths of light can be analyzed.