Laminar flow hoods protect the working environment from dust and other airborn contaminants by maintaining a constant, unidirectional flow of HEPA-filtered air over the work area.
How do cell culture hoods work?
Cell culture hoods, commonly Class II are used to create a sterile environment for cell culture experiments. These hoods protect the samples from contamination using air that is circulated within the enclosed bench and drawn through a HEPA filter.8 Oct 2019
What is the difference between fume hood and laminar flow hood?
The primary function of a fume hood is to protect the user from inhaling toxic substances. They also provide limited protection in the event of an explosion or spill. A laminar flow hood not only protects personnel but also protects the substance from contamination as well.
What is the difference between a laminar flow hood and a biosafety cabinet?
A Laminar Flow Hood (LFH), is not a biological safety cabinet. These devices do not provide any protection to the worker. They are designed to provide a sterile environment to protect the product. Air potentially contaminated with infectious agents may be blown towards the worker.
How do you turn on a tissue culture hood?
Use of Tissue Culture Hoods in Stem Cell Culture Facility 3.0 PROCEDURE 3.1 1) Ensure that sash on hood is completely closed. 2) Turn on blower and UV light, let them run for 15 minutes before using hood. 3) After 15 minutes, turn off UV light and raise sash to line marked on hood for use.16 Jan 2018
How do you decontaminate a tissue culture hood?
Wipe down all surfaces in the hood with soapy water and then 70% ethanol starting from the back and working your way forwards. Again, products that contain bleach are not ideal here as you do not want to damage the hood. Don't forget the sash and the outside of the hood.3 Jun 2016
How would you sterilize cell culture hood?
- GIVE FOLKS A HEADS UP THAT CLEANING DAY IS COMING UP.
- SET UP YOUR SPACE.
- TAKE ANY EQUIPMENT PERMANENTLY STORED IN THE HOOD OUT.
- DISMANTLE THE HOOD.
- SCRUB-A-DUB-DUB.
- HEAT WAVE IN THE AUTOCLAVE.
- BACK TOGETHER AGAIN.
How do you clean biosafety cabinets after use?
Decontaminate the BSC before and after every use to prevent cross-contamination. Use a 1:10 fresh bleach solution followed by a 70% ethanol rinse to avoid corrosion and achieve good disinfection, or contact EHS if you need a bleach alternative.
Which steps do you have to take when working under a sterile hood?
- Understand What Kind of Lab Hood Is Helping Keep You and Your Cells Contamination Free.
- Keep the Lab Stocked with Bleach and Ethanol.
- Plan Your Waste Management.
- “Spray as You Go in, Spray as You Go out”
- Organize Your Workspace Strategically.