Abstract. Viral fusion proteins are essential for enveloped virus infection. These proteins mediate fusion between the virus envelope and host cellular membrane to release the viral genome into cells. Vesicular stomatitis virus G (VSV G) protein is a typical type III viral fusion protein.13 Jul 2018
What does VSV G bind?
VSV genome encodes five structural proteins among which a single-transmembrane glycoprotein (G). G plays a critical role during the initial steps of virus infection4. First, it is responsible for virus attachment to specific receptors. After binding, virions enter the cell by a clathrin-mediated endocytic pathway5,6.12 Mar 2018
How does VSV g enter cells?
After binding to the cell surface, VSV particles enter the cell through endocytosis, in a clathrin-based, dynamin-2-dependent manner (Superti et al., 1987). The virus can enter either through a preformed clathrin-coated pit (CCP) or by de novo induction of pit formation (Johannsdottir et al., 2009).22 Jun 2013
What type of virus is VSV?
17.7 Vesicular Stomatitis Virus (VSV) VSV is another single-stranded RNA virus of the rhabdoviridae family, which includes the rabies virus. Because it is closely related to rabies in structure and biology, yet less pathogenic, it has been studied as a proxy to understanding rabies biology.
What does VSV G do?
VSV-G envelope glycoprotein forms complexes with plasmid DNA and MLV retrovirus-like particles in cell-free conditions and enhances DNA transfection. Mol Ther. 2001 Sep;4(3):232-8.
What do LDL receptors bind to?
LDL-R binds LDL at neutral pH on the cell surface of hepatocytes. The ligand-receptor complex internalizes through receptor-mediated endocytosis at clathrin-coated pits, releasing its cargo in the endosomes upon exposure to acidic pH (2).11 Aug 2009
How does VSV enter cells?
For the vesicular stomatitis virus (VSV), the receptor is ubiquitous, but unknown, and virus enters cells via clathrin-mediated endocytosis, with fusion occurring with endosomes at a pH of approximately 5.8.
What is vesicular stomatitis virus glycoprotein?
Glycoprotein G of the vesicular stomatitis virus (VSV) is involved in receptor recognition at the host cell surface and then, after endocytosis of the virion, triggers membrane fusion via a low pH-induced structural rearrangement.
What is the receptor for VSV G?
LDL
What is VSV G virus?
Vesicular stomatitis virus G (VSV G) protein is a typical type III viral fusion protein. To study the mechanism of VSV G protein mediated membrane fusion, we set up a cell-cell fusion system in which cells are marked by different fluorescent proteins.Vesicular stomatitis virus G (VSV G) protein is a typical type III viral fusion protein. To study the mechanism of VSV G protein mediated membrane fusion, we set up a cell-cell fusioncell-cell fusionCell fusion is an important cellular process in which several uninucleate cells (cells with a single nucleus) combine to form a multinucleate cell, known as a syncytium. Cell fusion is a necessary event in the maturation of cells so that they maintain their specific functions throughout growth.https://en.wikipedia.org › wiki › Cell_fusionCell fusion - Wikipedia system in which cells are marked by different fluorescent proteins.13 Jul 2018
Is vesicular stomatitis bacterial or viral?
Vesicular stomatitis is a viral disease that creates ulcerations of the oral mucosa in cattle, swine, and horses.
What pathogen causes vesicular stomatitis?
Vesicular stomatitis can be caused by four named viruses in the genus Vesiculovirus (family Rhabdoviridae): vesicular stomatitis New Jersey virus (VSV-NJ), vesicular stomatitis Indiana virus (VSV-IN), vesicular stomatitis Alagoas virus (VSV- AV) and Cocal virus.21 Jan 2016
Is VSV an adenovirus?
dl-VSVG-LacZ, a vesicular stomatitis virus glycoprotein epitope-incorporated adenovirus, exhibits marked enhancement in gene transduction efficiency. Hum Gene Ther. 2003 Nov 20;14(17):1643-52. doi: 10.1089/104303403322542284.