Dissolve salmon sperm DNA (Sigma type III sodium salt) in H2O at a concentration of 10 mg/mL. If necessary, stir the solution on a magnetic stirrer for 2-4 h at room temperature to help the DNA to dissolve.
What is salmon sperm DNA used for?
Salmon Sperm DNA Solution was developed for use in hybridization protocols as a blocking agent to reduce the non-specific binding of a hybridization probe to the surface of the filter. Carrier DNA is typically used at a concentration of 100 µg/ml in both the prehybridization and hybridization solutions.
How is salmon sperm DNA prepared?
Description: Salmon Sperm DNA Solution is prepared from highly pure, phenol- chloroform extracted DNA, and DNase-free, RNase-free (DEPC-treated), distilled, deionized water. Once dissolved, the DNA solution is sheared to an average size of ≤ 2000 bp and the concentration is adjusted to 10 mg/ml.